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Data Sheets

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Diagnostics

Introducing the BioCell Protein Carbonyl Assay Kit

In vitro marker of oxidative injury.

Diagnostics image

The BioCell Protein Carbonyl Assay Kit is an enzyme-linked immuno-sorbent assay (ELISA) for the measurement of protein carbonyls in biological samples. Each kit contains everything you need, including standards, control samples, all reagents, detailed instructions and plate.

Key Benefits:

  • Measures carbonyls quantitatively
  • Requires only microgram amounts of protein
  • Has a very low non-specific background
  • Is highly sensitive for analysing clinical samples
  • Correlates well with the colorimetric assay
  • Easier to use and less labour intensive
  • Can handle more samples per day than colorimetic assays.

Assay Principle:

Protein samples are reacted with DNP then non-specifically adsorbed to an ELISA plate. Free DNP and non-protein constituents are easily washed away, meaning minimal interference.

The adsorbed protein is reacted with a biotinylated anti-DNP antibody followed by streptavidin-biotinylated horseradish peroxidase. Absorbances are related to a standard curve prepared from serum albumin containing increasing proportions of HOCl-oxidized protein.

Using the kit:

  • Up to 81 samples can be analysed in single analyses, although duplicate or triplicate analysis is recommended for best results.
  • The kit components can be used more than once, permitting the analysis of fewer samples at a time without using all of the kit at once.
  • Carbonyl concentrations are calibrated against a protein that has been oxidized and standardized colorimetrically.

Stability:

The reagents in this kit will be stable for at least one year if stored as indicated. BioCell Protein Carbonyl Assay Kits do not need to be kept under cool conditions for short-term transportation. The kits are stable at room temperature for up to six weeks.

Background:

An ELISA test, using an anti-DNP antibody, was developed by: I. Hendrikje Buss, Christine C. Winterbourn and coworkers at the Free Radical Research Group, Christchurch School of Medicine (University of Otago), Christchurch, New Zealand.

References:

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