Data Sheets
The following "pdf" files contain product specific information and may be opened with acrobat reader 3.0 or higher.
- PC Kit Performance Information
- PC Kit Orders Ordering Details
- Kit Instructions Batch P092
- Batch P092 Certificate of Analysis
- Kit Instructions Batch P093
- Batch P093 Certificate of Analysis
- Kit Instructions Batch P094
- Batch P094 Certificate of Analysis
- Kit Instructions Batch P095
- Batch P095 Certificate of Analysis
- Kit Instructions Batch P096
- Batch P096 Certificate of Analysis
- Kit Instructions Batch P097
- Batch P097 Certificate of Analysis
- MSDS PC KIt MSDS
Protein Carbonyl Assay Kit
The BioCell Protein Carbonyl Assay Kit is an enzyme - linked immuno-sorbent assay (ELISA) for the measurement of protein carbonyls in biological samples.
Protein carbonyls are formed through oxidation of proteins by a variety of mechanisms. They are sensitive markers of oxidative injury.
Key Benefits:
- Measures carbonyls quantitatively
- Requires only microgram amounts of protein
- Has a very low non-specific background
- Is highly sensitive for analysing clinical samples
- Correlates well with the colorimetric assay
- Is easier to use, a lot less labor-intensive and can handle more samples per day than the colorimetic assay
Assay Principle:
Protein samples are reacted with DNP then nonspecifically adsorbed to an ELISA plate. Free DNP and nonprotein constituents are easily washed away, giving minimal interference.
The adsorbed protein is reacted with a biotinylated anti-DNP antibody
followed by streptavidin-biotinylated horseradish peroxidase. Absorbances are related to a standard curve prepared from serum albumin containing increasing proportions of HOCl-oxidized protein.
How the Kit Works:
- Standards, control samples, all reagents, detailed instructions and plate are supplied.
- Up to 81 samples can be analysed in single analyses, although duplicate or triplicate analysis is recommended for best results. The kit components can be used more than once, permitting the analysis of fewer samples at a time without using up the entire kit at once.
- Carbonyl concentrations are calibrated against a protein that has been oxidized and standardized colorimetrically.
Stability:
The reagents in this kit will be stable for at least six months if stored as indicated.
PC Kits do not require to be kept under cool conditions for short term transportation. The kits are stable at room temperature for up to 6 weeks.
Frequently Asked Questions
Visit our FAQ page.
Background:
An ELISA test, using an anti-DNP antibody, was developed by: I. Hendrikje Buss, Christine C. Winterbourn and coworkers at the Free Radical Research Group, Christchurch School of Medicine (University of Otago), Christchurch, New Zealand.
References:
- Protein Carbonyl Measurement By A Sensitive ELISA Method
- Protein Carbonyl Measurements by Enzyme-linked Immunosorbent Assay
- Resistance Exercise Effects On Blood Glutathione Status and Plasma Protein Carbonyls
- Oxidative Protein damage Associated With poor Grip Strength Among Older Women
- Effects of Cranberry Powder on Serum Lipid Profiles and Biomarkers of Oxidative Stress in Rats
- Protective Effect Of Red Beetroot Against CCl4 and NDEA-Induced Oxidative Stress in Rats
- Protein Carbonyl Groups as Biomarkers of Oxidative Stress
- Carbonylated Proteins in BAL of Patients With Sarcoidosis.....
- Oxidative Stress and Severe Walking Disability Among Older Women